Table 1 RT-qPCR primer sequences
Gene | Sequences (5′ - 3′) |
|---|---|
LncRNA PVT1 | F: TGGTACCGAGCTCGGATCCTCAAGATGGCTGTGCC |
R: CCGCCACTGTGCTGGATGATAGAAAAAGAATTTAATAG | |
sirt6 | F: GCCGTCTGGTCATTGTCA |
R: AGCCTTGGGTGCTACTGG | |
Ki67 | F: ACCTACCTTCAACGCTCTCTGA |
R: TCCGCTTACTTCTGGACAATCT | |
PCNA | F: ACCGCTGCGATCGCAACCTG |
R: GGCATGTTTACTACGCAGCTG | |
Bax | F: ATGCGTCCACCAAGAAGC |
R: CAGTTGAAGTTGCCATCAGC | |
Bcl-2 | F: GACTGAGTACCTGAACCGGCATC |
R: CTGAGCAGCGTCTTCAGAGACA | |
caspase-3 | F: AAGCGTGCCAACGACAGCTGTGGGAC |
R: TTAATAGTCATCATATTGACACTGGC | |
GAPDH | F: TTGGTATCGTGGAAGGACTCA |
R: TGTCATCATATTTGGCAGGTT |