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Table 2 MSP primer sequences

From: Long non-coding RNA PVT1 knockdown suppresses fibroblast-like synoviocyte inflammation and induces apoptosis in rheumatoid arthritis through demethylation of sirt6

Gene

Sequences (5′ - 3′)

sirt6-methylation

F: GTGTAACGGAGTAAGGGATGTTAC

R: ACTAAACCCATATAAATTCACCGAA

sirt6-unmethylation

F: TGTGTAATGGAGTAAGGGATGTTAT

R: ACTAAACCCATATAAATTCACCAAA

  1. Note: MSP, methylation specific polymerase chain reaction; Sirt6, sirtuin 6; F, forward; R, reverse
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Journal of Biological Engineering

ISSN: 1754-1611