Fig. 4From: Construction of an immunotoxin via site-specific conjugation of anti-Her2 IgG and engineered Pseudomonas exotoxin APurification of the trastuzumab-PE24 conjugate. The mono-conjugated trastuzumab-PE24 conjugate was purified by two-step purification using size exclusion and anion exchange chromatography. The full image of Fig. 4 is shown in Additional file 3bBack to article page