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Fig. 5 | Journal of Biological Engineering

Fig. 5

From: Construction of an immunotoxin via site-specific conjugation of anti-Her2 IgG and engineered Pseudomonas exotoxin A

Fig. 5

(a) ADP-ribosylation of eEF-2. Wheat germ extract including eEF-2 was incubated with biotinylated NAD+ in the presence of PE24-AzF or trastuzumab-PE24, and then the reactions were analyzed by western blot using streptavidin-HRP conjugate. b Binding analysis of trastuzumab and trastuzumab-PE24 on Her2 antigen by ELISA. The analyses were done three times. The error bars represent one standard deviation

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Journal of Biological Engineering

ISSN: 1754-1611