Table 1 Primer sequences for RT-qPCR
Gene | Sequences (5′-3′) | |
|---|---|---|
miR-138-5p | F: GCCGGATAAGTGTTGTGGTCGA | R: ACTGAGCAAGCACTACCACCAGCA |
LCN2 | F: TGCCACTCCATCTTTCCTGTT | R: GGGAGTGCTGGCCAAATAAG |
GAPDH | F: TTCACCACCATGGAGAAGGC | R: GGCATGGACTGTGGTCATGA |
Caspase-3 | F: TGTCATCTCGCTCTGGTACG | R: AAATGACCCCTTCATCACCA |
Bax | F: TGCAGAGGATGATGCTGAC | R: CCTGTGACCTGAAGGAGG |
Bcl-2 | F: CCTGTGCCACCATGTGTCCATCC | R: GCTGAGAACAGGGTCTTCAGAGAC |
U6 | F: CTCGCTTCGGCAGCACA | R: AACGCTTCACGAATTTGCGT |
IL-6 | F: CCACTTCACAAGTCGGAGGCTT | R: CCAGCTTATCTGTTAGGAGA |
IL-1β | F: GAAACAGCAATGGTCGGGAC | R: AAGACACGGGTTCCATGGTG |
TNF-α | F: CGGAGTCCGGGCAGGT | R: GCTGGGTAGAGAATGGATGAACA |