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Fig. 10 | Journal of Biological Engineering

Fig. 10

From: An overview of advanced biocompatible and biomimetic materials for creation of replacement structures in the musculoskeletal systems: focusing on cartilage tissue engineering

Fig. 10

Fluorescent immunocytochemistry tests and quantification of Pax7 and MyoD. (a) Muscle stem cells in Matrigel and collagen-I coatings were stained for Pax7 or MyoD (both green) and DAPI (blue). (b) Quantification of Pax7+ and MyoD+ cells (expressed as a mean ± SD) in Matrigel and collagen-I coatings. (c) Indirect quantification of the number of cells (expressed as a mean ± SD) in Matrigel and collagen-I coatings. Scale bar represents 100 μm. Significant difference between collagen-I and Matrigel. Republished with permission of ref. [112], Grefte S, Vullinghs S, Kuijpers-Jagtman A, Torensma R, Von den Hoff J. Matrigel, but not collagen I, maintains the differentiation capacity of muscle-derived cells in vitro. Biomedical materials. 2012;7(5):055004, Copyright (2019)

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