Fig. 10

Fluorescent immunocytochemistry tests and quantification of Pax7 and MyoD. (a) Muscle stem cells in Matrigel and collagen-I coatings were stained for Pax7 or MyoD (both green) and DAPI (blue). (b) Quantification of Pax7⁺ and MyoD⁺ cells (expressed as a mean ± SD) in Matrigel and collagen-I coatings. (c) Indirect quantification of the number of cells (expressed as a mean ± SD) in Matrigel and collagen-I coatings. Scale bar represents 100 μm. ^∗ Significant difference between collagen-I and Matrigel. Republished with permission of ref. [112], Grefte S, Vullinghs S, Kuijpers-Jagtman A, Torensma R, Von den Hoff J. Matrigel, but not collagen I, maintains the differentiation capacity of muscle-derived cells in vitro. Biomedical materials. 2012;7(5):055004, Copyright (2019)