Fig. 5

Comparison between the 3D-tip and the centrifugation methods for fluorescence staining of fixed 3D OEC cultures. a Representative images of fixed 3D OEC spheroids stained with Hoechst (blue) and CellMask Deep Red (red) using the 3D-tip and the centrifugation methods. b Sphericity analysis of 3D structures after fluorescence staining using the 3D-tip and the centrifugation methods. c Percentage of spheroids remaining after fluorescence staining using the 3D-tip and the centrifugation methods. Scale bar = 50 μm. Images were captured on an Olympus IX73 microscope. The analysis was performed using AnaSP 1.0. p*< 0.05, n = 3, technical replicates