Fig. 3
From: Evaluation of human primary intestinal monolayers for drug metabolizing capabilities
Evaluation of brush border enzyme activity. ALP (a) and AAP (b) activity of cells cultured on the gradient cross-linked and conventional scaffold on days 5, 8 and 10 of culture (n = 3). U = μmol/min/mL. Shown is the average and standard deviation of the data (n = 3). In panel (a), 1 U is equal to the concentration (mM) of pNPP hydrolyzed after 60 min. For AAP activity, 1 unit is equal to the μM of L-Ala-NA hydrolyzed to L-alanine and p-nitroaniline per minute