Fig. 4

Impact of stiffness on YAP behavior. a YAP immunofluorescence (green) of monolayers at days 0, 1 and 2 after placement of the cells on the gradient cross-linked or conventional scaffold. DNA was counterstained with Hoechst 33342 (blue). Scale bar = 50 μm. b Quantification of the average fluorescence of YAP per cell nucleus for cells on the gradient cross-linked or conventional scaffold at days 0, 1 and 2 of culture. c CYP3A4 activity was measured for monolayers at day 10 (n = 4) after the cells were treated with verteporfin (YAP inhibitor) from days 0–2 and with rifampicin (CYP3A4 inducer) from days 8–10. Shown is the average and standard deviation of the data (n > 3)