Fig. 5

Protein sequence alignment of KcINU1 with three other GH32 members from fungi, including S.cerevisiae invertase (ScINV, PDB code 4EQV), Aspergillus awamorii exo-inulinase (AaEI, PDB code 1Y9G), and Xanthophyllomyces dendrorhous β-fructofuranosidase (XdINV, PDB code 5ANN). Convinced glycosylation sites were highlighted in yellow. The black asterisks indicated residues involved in the catalytic center, and the red squares indicated amino acid similarity as calculated by ESPript. The structural annotation was based on the crystal structure of ScINV