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Fig. 4 | Journal of Biological Engineering

Fig. 4

From: CRIMoClo plasmids for modular assembly and orthogonal chromosomal integration of synthetic circuits in Escherichia coli

Fig. 4

Orthogonality of reporter gene expression between different integration sites and between different resistance cassettes used for integration. (a) Correlation graphs between the luciferase activities in Fig. 3a, obtained from PBAD-lux integrated into different att sites. Each data point represents mean and standard deviation measured at the same time point in Fig. 3a, while the color code indicates the inducer concentration as in Fig. 3. (b) Correlation graphs between luciferase activities obtained from PBAD-lux integrated into attHK022, using CRIMoClo plasmids with four indicated resistance cassettes (chloramphenicol, kanamycin, spectinomycin, gentamicin). All data indicate averages from at least two independent biological assays and error bars denote standard deviations

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