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Table 1 Predicted PCR product sizes for attB sites, using primers P1-P2-P3-P4. Successful integration events at each attB site are revealed by two fragments generated by P1-P2 and P3-P4 (highlighted in green). Recombinants with two (or more) CRIMoClo plasmids at the attB site show in addition a third fragment generated by P2-P3. False positive (non-integrants) are revealed by the PCR product generated by P1 to P4 (highlighted in red)

From: CRIMoClo plasmids for modular assembly and orthogonal chromosomal integration of synthetic circuits in Escherichia coli