Fig. 3
From: A novel approach for T7 bacteriophage genome integration of exogenous DNA
Integration assay. a. Plague formation assay with diluted lysate from integration product. b. Recombinants detect PCR with universal primer pair Int-SP3 and G11-SR. Expected product is 518 bp. Panel 1–5 indicate negative control group lysates in quintuplicates that contain no pMCBK but pEXM4. Panel 6–10 indicate experimental group lysates in quintuplicates that contain both pEXM4 and pMCBK. Panel 11–23 indicate 13 isolated candidate recombinants from plague formation assay. Panel 24 indicates Blank contral with deionized water as PCR template