Fig. 5
From: A novel approach for T7 bacteriophage genome integration of exogenous DNA
Validation of longer insert integration. a. Detect PCR of candidate CE3-integrated and CE4-integrated recombinants. EP represents the ensemble primer pair InsertSeq-F1 and InsertSeq-R1. The correspongding products in CE3-integrated and CE4-Integrated recombinants were 2.89 kbp and 3.549 kbp respectively. UP represents the universal primer pair Int-SP3 and G11-SR with an expected product of 518 bp. B represents Blank contral with deionized water as PCR template. b. Gene structure of CE4-integration reaction product was analyzed after sequencing. Red solid rectangle represents T7 promoter