Fig. 8From: Formaldehyde formation in the glycine cleavage system and its use for an aldolase-based biosynthesis of 1,3-propanediolIn vitro 1,3-PDO synthesis. G1: glycine and pyruvate as substrates via GCS coupled with the enzymes KHG, KDC, and DhaT; G2: formaldehyde (5 mM) and pyruvate as substrates with only the three enzymes KHG, KDC, and DhaT; G3: glycine as substrate catalyzed by GCS without adding KHG, KDC, and DhaTBack to article page