Fig. 1
Glucose release per well of fed-batch MTPs into Wilms-MOPS medium with modified matrix composition. The left Y-axis represents the absolute amount of released glucose per well and the right Y-axis represents the measured glucose concentration in the experiment per well with VL,48 = 1000 μL/well. Grey data points correspond to the release of a 48-round-well fed-batch MTP with the standard matrix investigated in a previous study of our laboratory [14]. All data points represent the mean values of measurements of three individual wells. Error-bars indicate the respective standard deviation. General experimental conditions: 48-round-well MTP, temperature T = 37 °C, humidity = 80%, shaking frequency n = 970 rpm, shaking diameter d = 3 mm, VL,48 = 1000 μL/well. No initial glucose was provided in the medium. No microorganisms were inoculated, thus only the sterile medium was filled into the wells. To avoid unintended growth of contaminants, 0.2 g/L NaN3 was added to the medium