From: FimH as a scaffold for regulated molecular recognition
FimH Variant | Mutation(s) | Penta-His KD (nM) w/o effector | Penta-His KD (nM) with effector | Observed regulation by effectors | Effect of conformation on binding to Penta-His |
---|---|---|---|---|---|
CDR1-6XH-L | 11–16➔6xH | 170 ± 16a | N/A | Higher affinity in tight conformation | |
114 ± 9b | 28 ± 3 manb | activated | |||
CDR1-6XH-T | 11–16➔6xH A188D | 38 ± 4a | 32 ± 3 mab21a | no effect | |
34 ± 3b | 13 ± 1 manb | activated | |||
CDR2-6xH-L | 47–53➔6xH | 7.5 ± 0.4a | N/A | Higher affinity in loose conformation | |
N/A | N/A | ||||
CDR2-6xH-T | 47–53➔6xH A188D/Y64R | 21 ± 1a | 51 ± 3 mab21a | inhibited | |
40 ± 9b | 120 ± 20 manb | inhibited | |||
CDR3-6xH-L | 135–140➔6xH | 2.8 ± 0.3a | N/A | No effect of conformation on affinity | |
N/A | N/A | ||||
CDR3-6xH-T | 135–140➔6xH A188D/Y64R | 3.6 ± 0.2a | 3.7 ± 0.2 mab21a | no effect | |
N/A | N/A |