Fig. 5

Tbe1 and Tbe2 effectively degrade TB and release butyrate. Measurement of butyrate production by tEc strains harbouring pBAD24::tbe1 (Tbe1) and pBAD24::tbe2 (Tbe2) are compared to that of tEc with empty pBAD24 (Control). Bacterial culture was supplemented with 5 mM TB and incubated for 24 h. Three independent experiments were performed, and values (means ± SEM) are displayed in each bar. **, P < 0.05 versus Control