Skip to main content

Table 6 Performance differences between static and perfusion reactor cultures

From: Lymphocyte expansion in bioreactors: upgrading adoptive cell therapy

Author - Year Expansion Purity Functionality changes
Fold Days Static control Fold Days
T cell
Lamers [124] - 1994 52.6 ± 21.3 14–17 Bag 238.4 ± 168.7 14–17 CD4/CD8 ratio = 0.51 ± 0.23 vs. CD4/CD8 = 0.44 ± 0.16 static culture NP
Trickett [127] - 2002 53.2 ± 20.1 7–8 Flasks 71.2 ± 42.8 7–8 NP NP
Jones [60] 2020 17.7 fold higher than static 9 Flask 9 Treg phenotype 93.7% for flasks versus 97.7% for reactor. Reactor cultures had 8-fold greater interleukin-10 stimulation index
TIL
Knazek [78] - 1990 124–1170 14–32 Bag No difference NP NP Bag and hollow fiber cultures has similar surface-antigen profiles; Cytotoxicity was similar in both systems
Hillman [115] - 1994 20–60 7 Plate 3 7 Shift in the T cell subpopulations is more pronounced in the bioreactor. NP
Freedman [119] - 1994 30.6 ± 5.6 18.2 ± 1.7 Plate, flask, bag 303.1 28.9 CD4/CD8 ratios do not have a statistically significant difference; no difference in proportions of CD16+ and CD56 NP