Fig. 6

Inverted brightfield and immunofluorescence microscopy of recellularized extracellular matrix (ECM) chips. ECM-chips were seeded with endothelialcolony forming cells (ECFC) in monoculture (a, b) or in coculture with rat (rMSC, c-k, m, n) or human mesenchymal stromal cells (hMSC, l, o, p) andincubated for 14 (a-d, g, h, m) or 23 days (e, f, i, j, k, l, n-p). H&E (a, c, e), DAPI (b, d, f), vWF (g, i) and CD31 (h, j, o) staining revealed confluent cellattachment on luminal ECM-chip side. A trend towards layered cell growth was observed in samples incubated for 23 days (e, f, i, j). CD90 (k, l, arrows),HNA (m, n, p) staining was observed. Scale bar represents 100 μm