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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: Engineered hypoxia-responding Escherichia coli carrying cardiac peptide genes, suppresses tumor growth, angiogenesis and metastasis in vivo

Fig. 2

Results of SDS-PAGE analysis. A Presence of a single band at about 5 kDa confirmed expression of cardiac peptides. Lane 1: protein marker, Lane 2: before induction of hypoxic condition, Lane 4: 24 h after induction of hypoxic condition. B The single band at 27 kDa relates to the molecular weight of GFP which is absent in the bacterial samples prior to induction of hypoxic condition. Lane 1: before induction of hypoxic condition, Lane 2: protein marker, Lane 3: 24 h after induction of hypoxic condition. C results of western blot analysis confirming the correct expression of GFP protein. Lane 1: protein marker, Lane 2: before induction of hypoxic condition, Lane 3: 24 h after induction of hypoxic condition

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