CRISPR/Cas9 mediated T7 RNA polymerase gene knock-in in E. coli BW25113 makes T7 expression system work efficiently

Table 3 Expression of sYFP under control of T7-LacI promoter in different bacterial strains

Strains	Plasmid	Expressed genes	Fluorescent signal
BW25113	pACYCD-sYFP	sYFP	63 ± 89
BW25113-T7	pACYCD-sYFP	sYFP	1402 ± 221
BL21(DE3)	pACYCD-sYFP	sYFP	5635 ± 43

ISSN: 1754-1611