Fig. 2
From: AZD2014, a dual mTOR inhibitor, attenuates cardiac hypertrophy in vitro and in vivo
Effects of AZD2014 on phenylephrine (PE)-induced cardiac hypertrophy. a The mRNA expression levels of cardiac hypertrophy marker ANP and BNP in cardiomyocytes treated as indicated were determined by qRT-PCR. #, p > 0.05; *, p < 0.05 vs. non-PE treated group. n = 3. b The protein expression levels of ANP in cardiomyocytes treated as indicated were determined by FACS (The gray shaded peak is the isotype control). c Representative fluorescence microscopy images of immunocytochemistry for cardiomyocyte marker Myh6 and ANP in cardiomyocytes treated as indicated. Scale bar = 25 μm. d Cell area and nucleus size in cardiomyocytes treated as indicated were determined by microscopy and Image J. Graphic representation of cell area and nuclear size from 20 randomly selected measured cells. #, p > 0.05; *, p < 0.05 vs. non-PE treated group. Scale bar = 25 μm