Table 3 Advantages and Disadvantages of enzymatic decellularization
From: Application of decellularized bone matrix as a bioscaffold in bone tissue engineering
Technique | Mechanism | Advantages | Disadvantages | Reference |
|---|---|---|---|---|
Enzymatic Decellularization | Proteases: trypsin: Breaks down cellular proteins on the c-side of Arg or Lys and then destroys ECM proteins such as collagen and elastin. Pepsine: Breaks the bounds between peptides Nucleases: Break sequences of nucleic acids. | highly effective | Can damage the proteins in the ECM, especially laminin and GAG It changes the structure of the matrix Further cleaning and enzyme removal is required They may promote immune response. | [75] |