Fig. 5

Histological and cellular analysis of BATs subjected to overload (8%) or physiological (4%) load elongation in comparison to unloaded controls. a Hematoxylin & eosin stain of longitudinal 5 μm slices. b Visualisation of the cytoskeleton in intact BATs by F-actin staining via Phalloidin (in green) and confocal laser scanning microscopy. Single z-stack images in 40x magnification are shown. Counterstaining was carried out using Hoechst 34580 staining dsDNA in the cell nucleus (blue). c Hematoxylin & eosin-stained micro slices were analysed for nuclear area, cell density and roundness (low values indicate an elongated shape). Dotted grey lines indicate mean value of unloaded controls