Fig. 3
From: Extracellular matrix dynamics: tracking in biological systems and their implications
Strategies for proteomic identification of NSPs. a Azide-tagged NSPs labeled via bioorthogonal approaches, such as BONCAT and glycosylation-enabled labeling, can be conjugated to affinity tags (e.g. biotin) via the click chemistry, and affinity-purified free from pre-existing proteins to enable ultrasensitive proteomic detection using MS. b Isotope-tagged NSPs exhibit a specific molecular weight shift compared to their untagged native counterparts (for example, each.13C-Arg tag increases the molecular weight by 6Â Da), facilitating the identification of MS peaks corresponding to the labeled NSPs