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Fig. 1 | Journal of Biological Engineering

Fig. 1

From: Cloning, expression, and one-step purification/immobilization of two carbohydrate-binding module-tagged alcohol dehydrogenases

Fig. 1

SDS-PAGE of E. coli M15ΔglyA final samples from well plate cultures in LB medium. A: His-ScADH. Lane M: molecular weight standard (kDa); lanes 1 to 10: induced cultures. B: CBM3-ScADH. Lanes 1 to 10: induced cultures; lane 11: negative control. C: CBM9-ScADH. Lanes 1 to 4: induced cultures; lane 5: negative control. Culture conditions: 2 mL LB, 24 °C, 24 h, 140 rpm, 0.4 mM IPTG. His-ScADH (37 kDa), CBM3-ScADH (55 kDa) and CBM9-ScADH (64 kDa) corresponding bands indicated with arrows

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Journal of Biological Engineering

ISSN: 1754-1611