Fig. 3From: Metabolic engineering of Escherichia coli BW25113 for the production of 5-Aminolevulinic Acid based on CRISPR/Cas9 mediated gene knockout and metabolic pathway modificationALA production in recombinant E. coli expressing various related genes. Cultivation was performed in 300 mL Erlenmeyer flask supplied with 30 mL modified minimal medium supplied with 10 g/L glucose for 24 h. Results are the average of three individual experiments. A final ALA accumulation result was measured. Adjusted P values were calculated using Dunnett's multiple comparisons test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)Back to article page