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Fig. 3 | Journal of Biological Engineering

Fig. 3

From: Metabolic engineering of Escherichia coli BW25113 for the production of 5-Aminolevulinic Acid based on CRISPR/Cas9 mediated gene knockout and metabolic pathway modification

Fig. 3

ALA production in recombinant E. coli expressing various related genes. Cultivation was performed in 300 mL Erlenmeyer flask supplied with 30 mL modified minimal medium supplied with 10 g/L glucose for 24 h. Results are the average of three individual experiments. A final ALA accumulation result was measured. Adjusted P values were calculated using Dunnett's multiple comparisons test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)

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