Fig. 5From: Metabolic engineering of Escherichia coli BW25113 for the production of 5-Aminolevulinic Acid based on CRISPR/Cas9 mediated gene knockout and metabolic pathway modificationGrowth characteristic of mutant E. coli strains in different medium. Cells were cultured in M9YE (A) or LB (B) medium with 50 μg/mL Kanamycin. Bacteria contained pET28b-ALA-LAA. Data are means of three replicatesBack to article page