Fig. 7From: Metabolic engineering of Escherichia coli BW25113 for the production of 5-Aminolevulinic Acid based on CRISPR/Cas9 mediated gene knockout and metabolic pathway modificationSchematic presentation ALA of production in E. coli via both C4 and C5 pathways. Glycine was added to the culture medium as a substrate for the C4 pathway. G6P, glucose-6-phosphate; Pyr, pyruvate; Ket, α-ketoglutarate; Glu, glutamate; GlutRNA, Glutamyl-tRNA; GSA, glutamate 1-semialdehyde aminotransferase; ALA, 5-aminolevulinic acid; PBG, porphobilinogenBack to article page