Table 1 Strains and plasmids employed in this study
Strain or plasmid | Relevant characteristic(s) | Source and/or reference |
|---|---|---|
Strain | ||
 E. coli DH5α | F-,φ80dlacZ ΔM15,Δ(lacZYA -argF)U169, deoR, recA1, endA1,hsdR17 (rK-, mK +), phoA, supE44, λ-, thi-1, gyrA96, rel | TransGen, Beijing |
 E. coli BW25113 | F-, DE(araD-araB)567, lacZ4787(del)::rrnB-3, LAM-, rph-1, DE(rhaD-rhaB)568, hsdR514 | Laboratory stock |
 E. coli BW25113-T7 (D0) | BW25113 int::(lacI::PlacUV5::T7 gene) ΔybhC | Laboratory stock |
 E. coli D1:F | BW25113-T7 ΔhemF | This study |
 E. coli D1:D | BW25113-T7 ΔdppA | This study |
 E. coli D1:M | BW25113-T7 ΔmppA | This study |
 E. coli D2:FY | BW25113-T7 ΔhemF, ΔybdK | This study |
 E. coli D2:FA | BW25113-T7 ΔhemF, ΔgdhA | This study |
 E. coli D2:FB | BW25113-T7 ΔhemF, ΔgadB | This study |
 E. coli D3:FYA | BW25113-T7 ΔhemF, ΔybdK, ΔgdhA | This study |
 E. coli D3:FYB | BW25113-T7 ΔhemF, ΔybdK, ΔgadB | This study |
 E. coli D3:FAB | BW25113-T7 ΔhemF, ΔgdhA, ΔgadB | This study |
 E. coli D4:FYAB | BW25113-T7 ΔhemF, ΔybdK, ΔgdhA, ΔgadB | This study |
 E. coli D5:FYABD | BW25113-T7 ΔhemF, ΔybdK, ΔgdhA, ΔgadB, ΔdppA | This study |
 E. coli D5:FYABM | BW25113-T7 ΔhemF, ΔybdK, ΔgdhA, ΔgadB, ΔmppA | This study |
 E. coli D6:FYABMD | BW25113-T7 ΔhemF, ΔybdK, ΔgdhA, ΔgadB, ΔmppA, ΔdppA | This study |
Plasmids | ||
 pUC57 | Cloning vector, AmpR, ColE1/pMB1/pBR322/pUC ori | Laboratory stock |
 pACYCD | Cloning vector, CmR, p15a ori | Laboratory stock |
 pET28b-ALA-LAA | plasmid for biosyntheizing ALA (f1 ori; KanR; hemA; hemL; eamA; LacI gene and T7-LacI promoter) | Laboratory stock |
 pUC-gltX-gltD | pUC57 containing gltX and gltD genes; J23107 promoter | This study |
 pUC-gltB-gltX | pUC57 containing gltB and gltX genes; J23107 promoter | This study |
 pUC-gltB-gltD | pUC57 containing gltB and gltD genes; J23107 promoter | This study |
 pUC-gltB-gltD-gltX | pUC57 containing gltB, gltD and gltX genes; J23107 promoter | This study |
 pCas | plasmid for CRISPR (temperature sensitive oriR101; KanR; the λ-Red operon under the control of arabinose-inducible promoter; S. pyogenes-derived cas9; sgRNA guided to ori-p15a under the control of lac operator) | Laboratory stock |
 pTarget-gene | plasmid for CRISPR (p15a ori; CmR; sgRNA guided to targeted gene, such as hemF, ybdK, gdhA, gadB, dppA or mppA ge | This study |
 pACYCD-gene | pACYCD containing targeted gene (such as hemF, ybdK, gdhA, gadB, dppA or mppA) | This study |
 pACYCD-Donor DNA | pACYCD containing Donor DNA for targeted gene (such as hemF, ybdK, gdhA, gadB, dppA or mppA) | This study |
 pACYCD-RS-hemA | pACYCD containing hemA from R. sphaeroides (amino acid sequence has been modified); T7-LacI promoter | This study |