Metabolic engineering of Escherichia coli BW25113 for the production of 5-Aminolevulinic Acid based on CRISPR/Cas9 mediated gene knockout and metabolic pathway modification

Table 4 Growth rate of these mutant strains in different medium

Strain	Growth Rate	Medium	Significant Summary
E. coli BW25113-T7 (D0)	0.246	LB
E. coli D1:F	0.271	LB	ns
E. coli D1:D	0.257	LB	ns
E. coli D1:M	0.278	LB	ns
E. coli D2:FY	0.305	LB	ns
E. coli D2:FA	0.238	LB	ns
E. coli D2:FB	0.252	LB	ns
E. coli D3:FYA	0.225	LB	ns
E. coli D3:FYB	0.242	LB	ns
E. coli D3:FAB	0.297	LB	ns
E. coli D4:FYAB	0.265	LB	ns
E. coli D5:FYABD	0.271	LB	ns
E. coli D5:FYABM	0.249	LB	ns
E. coli D6:FYABMD	0.287	LB	ns
E. coli BW25113-T7 (D0)	0.429	M9YE
E. coli D1:F	0.457	M9YE	ns
E. coli D1:D	0.510	M9YE	ns
E. coli D1:M	0.479	M9YE	ns
E. coli D2:FY	0.445	M9YE	ns
E. coli D2:FA	0.305	M9YE	**
E. coli D2:FB	0.343	M9YE	ns
E. coli D3:FYA	0.360	M9YE	ns
E. coli D3:FYB	0.335	M9YE	*
E. coli D3:FAB	0.430	M9YE	ns
E. coli D4:FYAB	0.404	M9YE	ns
E. coli D5:FYABD	0.404	M9YE	ns
E. coli D5:FYABM	0.406	M9YE	ns
E. coli D6:FYABMD	0.443	M9YE	ns

P values were calculated using Dunnett’s multiple comparisons test (*P < 0.05, **P < 0.01)

ISSN: 1754-1611