Fig. 3
Cultivation of G. oxydans 621H ΔhsdR pBBR1p264-FDH-ST in a μRAMOS device with 60 g/L fructose with different ammonium and amino acid concentrations. Depicted is the oxygen transfer rate (OTR). Cultivations were performed at 30 °C, 1000 rpm, VL = 500 μL in a 48-well round well microtitre plate at a shaking diameter of 3 mm, initial pH value: 6. AA: amino acids, crucial AA: glutamate, glycine and isoleucine. Media (with elemental nitrogen concentration): Complete Poolman medium as reference (black, 912 mgN/L) and without (NH4)2SO4 with all amino acids (pink, 700 mgN/L), with (NH4)2SO4 with crucial amino acids (purple, 345 mgN/L), with 1.5-fold (NH4)2SO4 with crucial amino acids (green, 443 mg/L N), with (NH4)2SO4 with crucial amino acids with tripled glutamate concentration (orange, 440 mgN/L). Mean values of at least 4 replicates are shown. The shadows around the curves indicate the standard deviation. For clarity, only every fifth measuring point is marked as a symbol