Fig. 2

Pyroptosis facilitates UHMWPE particle-induced calvarial osteolysis. A western blot analysis of caspase-1, IL-1β, and GSDMD in calvarial treated with UHMWPE particles and/or Ac-YVAD-CMK. B Bar charts showing the densitometric analysis of western blot shown in A. C&D The protein level of IL-1β and the relative LDH activity in culture supernatant of calvarial tissues (n = 3). E Representative micro-CT three-dimensional reconstructed images of calvarial. Dotted red lines indicate the region of interest (n = 3). F Bar charts showing the total volume of pore space in calvarial shown in E. G&H BMD and BV/TV in the region of interest shown in (E) were measured (n = 3). I&J H&E staining (I) and TRAP staining (J) of longitudinal sections of calvarial (n = 3), scales bars represent 400 μm. The rightmost pictures designate the larger magnification of the regions shown in inset, scales bars represent 100 μm. K&L Bar charts showing the histological score and integrated optical density (IOD) value of TRAP positive area shown in (I&J). *P < 0.05, **P < 0.01, ***P < 0.001. P-values were analyzed by one-way ANOVA in (C&D, G&H), two-tailed t tests in (F, K&L). All data are representative of two to three independent experiments