Fig. 6
DFO alleviates UHMWPE particle-induced osteocytic pyroptosis. A Western blot analysis of caspase-1, IL-1β, and GSDMD in calvarial treated with UHMWPE particles and/or DFO. B Bar charts showing the densitometric analysis of western blot shown in (A). C&D The protein level of IL-1β and the relative LDH activity in culture supernatant of calvarial tissues (n = 3 or 4). E, G, I, K TUNEL staining, caspase-3 and caspase-1 IHC staining and H&E staining of longitudinal sections of calvarial (n = 3), scales bars represent 25 μm. F, H, J Bar charts showing the percentage of TUNEL, caspase-3 or caspase-1 positive cells shown in (E, G, I). L&M Bar charts showing the percentage of osteocytes with empty lacunae and the number of osteocytes with empty lacunae per mm2 shown in (K). O Western blot analysis of caspase-1, IL-1β, and GSDMD in MLO-Y4 cells induced by UHMWPE particles and/or DFO. P Bar charts showing the densitometric analysis of western blot shown in (O). Q&R The protein level of IL-1β and the relative LDH activity in culture supernatant of MLO-Y4 cells. *P < 0.05, ***P < 0.001. P-values were analyzed by one-way ANOVA in (B-D, P-R) and two-tailed t tests in (F, H, J, L, M). All data are representative of two to three independent experiments