Fig. 2
From: Mixed culture biocatalytic production of the high-value biochemical 7-methylxanthine
Mixed-culture resting cell reactions consumed caffeine and generated 7-methylxanthine. Equal concentrations of pADP1 and pBDP1 cells were reacted with the following initial caffeine concentrations and at the following optical densities: A, D 5 mM caffeine, OD600, total = 50 (●, orange) & OD600, total = 25 (▼, green), B, E 2 mM caffeine, OD600, total = 20 (■, teal) & OD600, total = 10 (▲, red), C, F 1 mM caffeine, OD600, total = 10 (♦, pink) & OD600, total = 5 (◊, blue). A-C represent the caffeine consumed, and D-F represent the 7-methylxanthine generated. All reactions were conducted in triplicate, and the representative data points are averages of the concentrations at a given time point with the corresponding standard deviation