Mixed culture biocatalytic production of the high-value biochemical 7-methylxanthine

Table 1 Comparison of Concentrations of Caffeine Consumed and Compounds Produced by Varied Cell Densities of pADP1 and pBDP1^a

OD₆₀₀ (NdmA/NdmB)	Caffeine Consumed (μM)	Theobromine Produced (μM)	7-Methylxanthine Produced (μM)
100:0	1022 ± 19	988 ± 24	10 ± 5
75:25	961 ± 64	545 ± 17	315 ± 27
50:50	643 ± 25	24 ± 11	667 ± 19
25:75	493 ± 43	-	481 ± 48
0:100	62 ± 57	-	22 ± 2

^aReported concentrations are averages of triplicate 1 mL reactions sampled at the conclusion of a 5-hour reaction initiated by the addition of 1 mM caffeine

ISSN: 1754-1611