Table 3 Complete List of Plasmids and Strains Used in this Study
From: Mixed culture biocatalytic production of the high-value biochemical 7-methylxanthine
Name | Characteristics | Source |
|---|---|---|
Plasmids | ||
pET-32a(+) | AmpR, T7 promoter, C-terminal His6 tag, pBR322 origin | Novagen |
dDA | pACYCDuet-1 with one copy of ndmD and one copy of ndmA | [27] |
dDB | pACYCDuet-1 with one copy of ndmD and one copy of ndmB | [29] |
dAA | pACYCDuet-1 with two copies of ndmA | [27] |
dBB | pACYCDuet-1 with two copies of ndmB | [29] |
pAD3 | pET-32a(+) with ndmA and ndmD linked through a pETrbs2 | This study |
pBD3 | pET32a(+) with ndmB and ndmD linked through pETrbs2 | This study |
dDP1DP1 | pACYCDuet-1 with two copies of ndmDP1 | This study |
pADP1 | pET-32a(+) with one copy of ndmA and one copy of ndmDP1 | This study |
pBDP1 | pET-32a(+) with one copy of ndmB and one copy of ndmDP1 | This study |
Strains | ||
E. coli BL21(DE3) | F- ompT hsdSB (r-Bm-B) gal dcm (DE3) | Invitrogen |
E. coli pADP1 | BL21(DE3) pADP1 | This study |
E. coli pBDP1 | BL21(DE3) pBDP1 | This study |
E. coli pAD3dDB | BL21(DE3) pAD3 dDB | This study |
E. coli pAD3dBB | BL21(DE3) pAD3 dBB | This study |
E. coli pBD3dAA | BL21(DE3) pBD3 dAA | This study |
E. coli pBD3dDA | BL21(DE3) pBD3 dDA | This study |
E. coli pADP1dBB | BL21(DE3) pADP1 dBB | This study |
E. coli pBDP1dAA | BL21(DE3) pBDP1 dAA | This study |
E. coli pAD3dDD | BL21(DE3) pAD3 dDD | This study |
E. coli pBD3dDD | BL21(DE3) pBD3 dDD | This study |