Fig. 1

Widefield microscopy in wide 3D migration chambers. (A). Representation of a wide migration chamber for chemotaxis assays. (B). Widefield image of VAL cells scattered in the field of view using TL. i-iii challenges for the automatic analysis: (i) large pixel size associated with a limited number of pixels per cell, (ii) appearance of cells similar to the background associated with a low signal to noise ratio, and (iii) different appearance of cells according to the position along the z-axis with respect to the focal plane