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Fig. 4 | Journal of Biological Engineering

Fig. 4

From: Tracking unlabeled cancer cells imaged with low resolution in wide migration chambers via U-NET class-1 probability (pseudofluorescence)

Fig. 4

Enhanced tracking of MDA-MB-231 breast cancer cells with heterogeneous shapes (A). Representative micrographs of the image pairs used to train the network. (B). Micrographs representing images of cells with heterogeneous shapes (round, or thin-elongated) in transmitted light, pseudofluorescence, and merged. (C). Comparison between tracks obtained using transmitted light (left) or pseudofluorescence (right). Arrows indicate tracking errors obtained using the transmitted light signal only. (D). Comparison in track duration using transmitted light vs pseudofluorescence signal with the proposed protocol. n = 76 cells (TL), 80 cells (PF). Statistics performed with Mann-Whitney t-test, *** = P < 0.001

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Journal of Biological Engineering

ISSN: 1754-1611