Fig. 4

T7Max performance characterization. Translation of GFP protein from circular plasmid template was measured at different temperatures and with different T7 RNA polymerase concentration. All green bars: T7 promoter, all blue bars: T7Max promoter. a expression of GFP measured after an 8-hour reaction at different temperatures. b RT qPCR measuring abundance of GFP mRNA in samples from panel a. c mRNA abundance measured at different times during the TxTl reaction at 30°C. d expression of GFP measured after an 8 hour reaction with different concentration of T7 RNA polymerase, TxTl reaction at 30°C. The percentage numbers above bars show fluorescence relative to the value at 2.5μM T7 RNAP for each promoter. All samples in triplicate, error bars represent standard deviation. The concentration of T7 RNAP was varied by adding different amounts of 25μM stock of overexpressed, purified T7 RNA polymerase, stock in 50% glycerol