Fig. 6

Synthetic minimal cells expressing GFP protein. Microscope images showing liposomes encapsulating plasmid encoding GFP under T7 (panels a and c) and T7Max (panels b and d) promoters. Panels a and b: 0.1mM lipid concentration, green (GFP) and red (rhodamine membrane dye) channels overlayed. Panels c and d: bright field showing density of liposomes at 10mM lipid. Scale bar is 5μm. e and f: quantification of 5 images taken from different fields of view in samples at 0.1mM lipid (panel e) and at 10mM lipid (panel f). Error bars represent standard deviation. The value is ratio of total fluorescence in green channel to total fluorescence in red channel