Table 2 Comparison of different cell separation methods
Technology | Pro | Contra |
|---|---|---|
LIFTOSCOPE | ▪ Rapid imaging ▪ Overview of the entire sample ▪ High transfer precision ▪ Functions can be used individually | ▪ Still in research ▪ Large investment if no microscope exists to upgrade |
FACS | ▪ Industry standard ▪ High throughput | ▪ Cells need to be transferred into liquid sample ▪ Disturbes the cells ▪ Unsuitable for small sample volumes due to large dead volumes ▪ Fluorescence labelling necessary |
Automated cell pickers | ▪ High control ▪ Precise cell uptake | ▪ About 20s per cell transfer |
Inkjet printer | ▪ Good control over cell number and deposition | ▪ Cannot be used in cell cultures directly ▪ Large dead volumes ▪ Shear forces at exit nozzle negatively impact cell viability |
LMPC | ▪ Overview of the entire sample ▪ Transfer of large samples | ▪ Can only be used with fixated samples ▪ Not suitable für single cell applications |