Fig. 1
Time-resolved (A-C) online monitoring signals and (D-F) offline sample measurements of H. polymorpha RB11 pC9-FMD (PFMD-GFP) cultivations at different initial cell dry weight (CDWt0) and magnesium (Mg2+) concentrations. (A) The mean oxygen transfer rate (OTR) of culture replicates (n = 2–3, Additional file 1:Fig. S3) was determined by a μRAMOS device [48]. The low standard deviations are shown as shaded areas and indicate good reproducibility. Hollow symbols indicate every sixth data point. (B) Scattered light (λex = λem = 600 nm) and (C) GFP fluorescence intensities (λex = 420 nm, λem = 530 nm) were extracted from 2D spectra of duplicates (solid and dotted lines). Hollow symbols indicate every fifth data point. Values of (D) glycerol, (E) cell dry weight (CDW), and (F) pH value are based on singular offline measurement from parallel cultivation, taken every 1.5 h (hollow and filled symbols). Filled, linearly interpolated symbols describe a sampling interval of 6 h. Cultivation conditions: 48-well microtiter plate with round geometry, modified SYN6-MES medium, liquid volume = 800 μL, shaking diameter = 3 mm, shaking frequency = 1000 rpm, temperature = 30 °C