Fig. 2
(A-C) Calibration and (D-F) prediction of the PLS models for three offline parameters for H. polymorpha RB11 pC9-FMD (PFMD-GFP) cultivations at different initial cell dry weight (CDWt0) and magnesium (Mg2+) concentrations. This figure is based on the data in Fig. 1. The PLS models were calibrated using the linearly interpolated values of the filled symbols and a total of 248 2D spectra of the two cultivation conditions shown in A-C. The PLS models for (A, D) glycerol, (B, E) CDW, and (C, F) pH value were generated using two, three, and two LVs, respectively. Solid and dotted lines describe the calculated parameter progression of duplicates. Hollow symbols in A-F were used for model validation only. Cultivation conditions: 48-well microtiter plate with round geometry, modified SYN6-MES medium, liquid volume = 800 μL, shaking diameter = 3 mm, shaking frequency = 1000 rpm, temperature = 30 °C