Fig. 5
Scores of first to third principal component (PC1-PC3) over cultivation time, based on (A-C) 2D spectra including scattered light and fluorescence, as well as based on (D-F) only fluorescence intensities for the cultivation of H. polymorpha RB11 pC9-FMD (PFMD-GFP) at different phosphate (PO43−) concentrations. The explained variance for each PC is shown in brackets. For clarity, only data of one replicate per cultivation condition is shown. Only every 10th datapoint is indicated by a symbol. Asterisks in Fig. 5A and E indicate reversed Y-axis direction used for clarity. Spectroscopic measurement settings: excitation wavelength range = 280 nm – 700 nm (step size = 10 nm), emission wavelength range = 278 nm – 720 nm (step size = 0.45 nm), integration time = 30 ms. Cultivation conditions: 48-well microtiter plate with round geometry, modified SYN6-MES medium, liquid volume = 800 μL, shaking diameter = 3 mm, shaking frequency = 1000 rpm, temperature = 30 °C