Unravelling the mechanotransduction pathways in Alzheimer’s disease

Table 3 Nuclear lamina components assessment

Result	Comparison	Method	Samples	REF
LAMIN A
↑	Healthy elderly case vs healthy young case	Immunofluorescence	Embryos from NMRI mice brain tissue	[63]
↑	Healthy elderly case vs healthy young case	Immunohistochemistry	Adult Sprague–Dawley rats (8 weeks old) brain tissue	[64]
↑↑	AD case vs healthy elderly case	Immunohistochemistry Immunofluorescence	Autopsied human AD brain tissue	[57]
↑↑	AD case vs both healthy young and healthy elderly cases	Western Blot	Human hippocampal samples	[58]
LAMIN B1
↓	Healthy elderly case vs healthy young case	Immunohistochemistry	Adult Sprague–Dawley rats (8 weeks old) brain tissue	[64]
↓	Healthy elderly case vs healthy young case	Immunocytochemistry	ICR mice or heterozygous GAD67-GFP knock-in mice at embryonic day 17.5 hippocampal tissue	[73]
↓	Healthy elderly case vs healthy young case	Immunocytochemistry	C57Bl/6 mice and human post-mortem brain material	[72]
↓	AD case vs healthy elderly case	Post-mortem Comparative Analysis (e.g. Western Blot)	Human brain tissue	[4]
↓	AD case vs healthy elderly case	Immunohistochemistry Western Blot 3D Confocal Microscopy	3xTg and APP/PS1 mouse models of AD and human post-mortem hippocampal tissue	[59]
LAMIN B2
↑	Healthy elderly case vs healthy young case	Immunohistochemistry	Adult Sprague–Dawley rats (8 weeks old) brain tissue	[64]
↑↑	AD case vs healthy elderly case	Immunohistochemistry Immunofluorescence	Autopsied human AD brain tissue	[57]

Results of the experiments about nuclear lamina conducted on post-mortem human brain samples and mice brain tissue. The variations of lamin A, B1 and B2 expression in aging and Alzheimer’s disease are reported with their related controls. The method, the samples and the reference are reported for each study. The arrows indicate the lamins concentration variation of healthy elderly case vs healthy young case and AD case vs healthy elderly case. “↑” and “↓” are used for an increase and a decrement, respectively. “↑↑” is used for a remarkable increment in lamin signal

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