Fig. 1

Establishment of ring culture system and experimental design. A Ring fabrication diagram showing the structure of PDMS-based culture system embedded with ring magnet. B Experimental design for hiPSC colony formation (Step 1) and self-organization of spatial patterns (Step 2) within the hiPSC colony with or without ring-barrier. The barrier made from detachable ring culture system is placed on culture surface and cells are seeded within this area; upon confluency, the barrier is removed, which results in a center and edge of the colony, a cell-free space that allows cell migration. In Step 2, the cell migration was monitored and the spatial self-organization within the hiPSC colonies was compared under culture conditions with and without HA exposure