Fig. 2

Spatial self-organization within the hiPSC colony with or without the ring-barrier. A Experimental design for hiPSC colony formation (Step 1) and self-organization of spatial patterns (Step 2) within the hiPSC colony with or without the ring-barrier. The barrier made from detachable ring culture system is placed on culture surface and cells are seeded within this area; upon confluency, the barrier is removed, which results in a center and edge of the colony, a cell-free space that allows cell migration. In Step 2, the cell migration was monitored and the spatial self-organization within the hiPSC colonies was compared under culture conditions with and without HA exposure. B Representative morphologies at different time points during spatial self-organization within hiPSC colony with or without ring-barrier are shown. The hiPSC colonies were compared after further exposure to HA in culture conditions without the ring-barrier. Colony diameter, 2.5 mm. Scale bar, 500 μm. C Representative images for cell morphology at the center and edge of the hiPSC colony in the image (B). All experiments were repeated independently at least three times with similar results