Fig. 7

Characterization of spatial self-organization within the hiPSC colony with or without the ring-barrier in the absence of exogenous differentiation factors. A Representative images in the XY plane showing the expression of E-cadherin and N-cadherin within the hiPSC colony. Panels a1–l1 are the tomograms sectioned at the XZ plane (yellow dashed lines) in panels a-l. Nuclei were stained with DAPI. Scale bar, 50 μm. B Representative images in the XY plane showing the expression of paxillin within the hiPSC colony. Panels m1–r1 are the tomograms sectioned at the XY plane (yellow dashed lines) in panels m-r. Nuclei were stained with DAPI. Scale bar, 50 μm. C A detailed description of the endodermal differentiation process within the hiPSC colony through collective cell migration. Differences in spatial self-organization within the hiPSC colonies in cultures with and without the ring-barrier removal are shown. All experiments were repeated independently at least three times with similar results